Reagents to power eProtein DiscoveryTM
Specially formulated reagents power your workflow from DNA preparation to protein expression screening, purifiability assessment and then off cartridge scale up.
Features and Benefits
The reagents within the eProtein DiscoveryTM system allow you to optimize protein obtainability by characterizing and purifying different combinations of DNA constructs and expression conditions. Our system will screen 192 different combinations in 24 hours for you to select the optimal conditions to scale up and get protein.
Our eProtein DiscoveryTM software will guide you in creating the panel of DNA constructs and reagents to power your experiment. Our complete reagent package includes design and ordering of DNA, simplifying your workflow.
eGene™ Prep Kits
Customize DNA constructs (eGene) design with pre-set fusion tags to optimize expression.
Cell-free Core Reagents & Additives
Optimize expression with different reagent formulations based on your protein needs.
Scale Up Kits
Scale up expression and purification of your proteins of choice to use in downstream applications.
Examples of proteins synthesized with eProtein Discovery™
Protein Class
Protein Name
- Interferon gamma (IFN-γ)
- Vascular endothelial growth factor (VEGF)
Technical Specifications
| eGene™ Prep Kit: Solubility tags | Multiple solubility tag options added to N-terminus |
| eGene™ Prep Kit: Purification tag | Strep added to C- terminus |
| eGene™ Prep kit: Protease cleavage sites | TEV and 3C protease sites added. Option for solubility screening after tag removal |
| Cell-free Core Reagents | E. coli derived Cell Free Protein Synthesis (CFPS) reagents |
| Additives: Redox regulators | Promote disulfide bond formation in your proteins |
| Additives: Chaperones | Aid in protein folding and stability |
| Additives: Metal ions | Promotes correct folding of metalloproteins |
| Additives: Co-factors | Stabilizes proteins |
| Purification reagents | Strep purification beads and purification reagents included |
| Scale Up Kits | Prototype-scale kits provided for scale up expression in tubes. Additional scale up material available to purchase |
Featured Resource
Webinar: Predictions to Proteins In-Hand in just 48hrs – Rapidly Express AlphaFold Predictions
The integration of AlphaFold AI within the eProtein Discovery framework brings forth a multitude of advantages, facilitating structure guided protein construct design with unprecedented precision.
Application Note: VEGF Expression
VEGF is a key regulator of angiogenesis and plays a central role in the process of tumor growth, making it an appealing target for anticancer therapeutics. Using the eProtein Discovery™ platform, we demonstrated that the ideal construct and conditions for expressing soluble VEGF can be determined within 24 hours.
